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RNAplant
植物RNA提取試劑
產(chǎn)品簡介:
RNAplant試劑可從植物組織,特別是富含多酚或淀粉的植物組織中(如馬鈴薯塊莖,白松松針或嫩苗,和西紅柿葉等)提取高純度的總RNA。
每100 ml可處理100 mg組織200次。
保存條件:室溫運輸,4℃保存,可保存6-12個月。
準備試劑:液氮,研缽,無RNase離心管,5 M NaCl(NC1050F 5M 氯化鈉 RNase-free,DNase-free),氯仿,異丙醇,75%乙醇(無RNase水配制),無RNase水。
小規(guī)模提取操作步驟:(樣品<0.1 g ,使用小規(guī)模提取方法):
1. 取不多于0.1 g植物組織,在液氮中研碎,轉(zhuǎn)移到1.5 ml 離心管中,加0.5 ml提取試劑,振蕩至徹底混勻。
注:振蕩不要太劇烈,否則容易導致基因組DNA污染。
2. 室溫放置5分鐘。
3. 4℃ 12, 000 rpm離心2-3分鐘,上清轉(zhuǎn)入新的無RNase離心管。
4. 上清中加入0.1 ml 5 M NaCl(NC1050F 5M 氯化鈉 RNase-free,DNase-free),溫和混勻。
5. 加入0.3 ml 氯仿,上下顛倒混勻。
6. 4℃ 12,000 rpm離心10-15分鐘,取上層水相轉(zhuǎn)入新的無RNase離心管。
7. 加與所得水相等體積的異丙醇,混勻,室溫放置10分鐘。
8. 4℃ 12,000 rpm離心10-15分鐘。棄掉上清,注意不要倒出沉淀。加1 ml 75%乙醇沖洗沉淀。(沉淀可能很難看見,應小心操作。)
9. 4℃ 5000-7000rpm離心3分鐘。倒出液體,注意不要倒出沉淀。剩余的少量液體短暫離心,用槍頭吸出,室溫晾干1-2分鐘至沉淀干燥。
10. 加適量無RNase水充分溶解RNA,-70℃保存。
注意事項:
RNAplant有刺激性味道,操作時請在通風處進行。
RNAplant植物RNA提取試劑(多糖多酚植物)(貨號:RTR2303)發(fā)表文章列表(13篇):
1. [2021
IF=4.7] The 5-untranslated region of the FAD3
mRNA is required for its translational enhancement at low temperature in Arabidopsis
roots.
Author: Chun-TaoWang, Yi-Nong Xu.
Journal: Plant
Science 179,234-240,2010
植物材料:擬南芥
Institution: Key
Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences,
Paper link:https://linkinghub.elsevier.com/retrieve/pii/S0168945210001470
2. [2021
IF=4.1] Different exogenous sugars affect the
hormone signal pathway and sugar metabolism in ‘‘Red Globe’’ (Vitis
vinifera L.) plantlets grown in vitro as shown by transcriptomic analysis.
Author: Juan Mao,Wenfang Li,Baoqin Mi,Zonghuan Ma,Yongmei Zhang,
Baihong Chen
Journal: Planta 246, 537–552,2017
植物材料:葡萄
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s00425-017-2712-x
3. [2021
IF=3.4] Transcriptome analysis revealed glucose
application afects plant hormone signal transduction pathway in “Red
Globe” grape plantlets s.
Author: Juan Mao ,Wenfang Li, Baoqin Mi, Zonghuan Ma,
Mohammed Mujitaba Dawuda, Cunwu Zuo
Journal: Plant Growth Regul volume
84, pages 45–56 (2018)
植物材料:葡萄
Institution:
College of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s10725-017-0320-1
4. [2021
IF=4.169] Effects of CEPA and 1-MCP on flower
bud differentiation of apple cv. 1 agafu No.2 grafted on different
rootstocks.
Author: Wenfang Li, Baihong Chen
Journal: J Plant Growth Regul. volume 38, pages 842–854 (2019)
植物材料:蘋果
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s00344-018-9895-7
5. [2021
IF=7.228] FIT and bHLH Ib transcription factors
modulate iron and copper crosstalk in Arabidopsis.
Author: Yuerong Cai, Yang Li, Gang Liang
Journal: Plant, Cell and
Environment. Volume44, Issue5 May 2021 Pages
1679-1691
植物材料:擬南芥
Institution: CAS
Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna
Tropical Botanical Garden
Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pce.14000
6. [2022
IF=6.2] Genome-Wide Identification and Abiotic
Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries.
Author: Lili Guo, Shixiong Lu, Tao Liu, Guojie Nai, Jiaxuan Ren, Huimin Gou, Baihong
Chen and Juan Mao
Journal: International Journal of Molecular
Sciences. 2023, 24,
4049.
植物材料:草莓
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.3390/ijms24044049
7. [2022
IF=2.561] Physiological and Transcriptomic
Analysis Reveals Drought?Stress Responses of Arabidopsis DREB1A in Transgenic
Potato
Author: Xiaoxia Jia, Enfang Qi, Shi Liu, Sheng Ma, Guohong Wen,
Xinhui Zhang, Heping Lv, Wei Huang, Xucheng Zhang
Journal: Potato Research Published
online 21 Feb 2023
植物材料:馬鈴薯
Institution: Gansu
Academy of Agricultural Sciences
Paper link:https://link.springer.com/article/10.1007/s11540-023-09619-7
8. [2022
IF=6.6] Molecular evolution and expression
assessment of DFRs in apple
Author: Wen?Fang
Li, Ju Gao, Zong?Huan
Ma, Ying?Jun
Hou, Xin Li, Juan Mao and Bai?Hong Chen
Journal: Chemical and Biological Technologies in
Agriculture (2023) 10:98
植物材料:蘋果
Institution: College
of Horticulture, Gansu Agricultural University
Paper link: https://doi.org/10.1186/s40538-023-00470-z
9. [2022
IF=3.5] Genome-wide identification and
expression analysis of the BURP domain-containing genes in Gossypium hirsutum
Author: Jiaxuan Ren,Li Feng,Lili Guo, Huimin Gou,Shixiong Lu,
Juan Mao
Journal: Physiology and Molecular Biology of
Plants 2023 29(11):1717–1731
植物材料:棉花
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.1007/s12298-023-01393-7
10. [2022
IF=2.9] Genome-wide identification and
expression analysis of the ZRT, IRT-like Protein (ZIP) family in Nicotiana
tabacum
Author: Zhijie Duan, Deka Reine Judesse Soviguidi, Bangzhen Pan, Rihua Lei,Zhongbang
Song, Gang Liang
Journal: Metallomics 2024
Oct
植物材料:煙草
Institution: Xishuangbanna
Botanical Garden,CAS
Paper link:https://pubmed.ncbi.nlm.nih.gov/39390668/
11. [2023
IF=3.9] Genome Wide Identifcation
and Expression Analysis of the NRL (NPH3/RPT2 Like)
Family in Vitis vinifera
Author: Yubin Li,Hong Pan, Yan Hao,Yanfang Zhu,
Qiang Chang, Jiaxuan Ren
Journal: Journal of Plant Growth Regulation Published
online 23 Oct 2024
植物材料:葡萄
Institution: Institute
of Forestry, Fruits and Floriculture, Gansu Academy
of Agricultural Sciences
Paper link:https://doi.org/10.1007/s00344-024-11530-5
12. [2023
IF=6.1] Genome-wide survey of chlorophyllase
(CLH) gene family in seven Rosaceae and functional characterization of MdCLH1
in apple (Malus domestica) leaf
photosynthesis.
Author: Wen-Fang
Li, Ji Liu, Tian-Yu Hu, Ying-Jun Hou, Zong-Huan Ma, Tong Feng, Zhi-Gang Guo,
Juan Mao, Bai-Hong Chen
Journal: Plant Physiology and Biochemistry 219 (2025) 109387
植物材料:蘋果
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.1016/j.plaphy.2024.109387
13. [2023
IF=4.9] Shading Treatment Reduces Grape Sugar
Content by Suppressing Photosynthesis-Antenna Protein Pathway Gene Expression
in Grape Berries.
Author: Xintong
Nan, Wenfang Li, Miao Shao, Zimeng Cui, Han Wang, Jiaxing Huo, Lizhen Chen,
Baihong Chen and Zonghuan Ma
Journal: International Journal of Molecular
Sciences 2024,
25, 5029.
植物材料:葡萄
Institution: College
of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.3390/ijms25095029
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