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Amphotericin B solubilized可溶性兩性霉素B

Amphotericin B solubilized可溶性兩性霉素B

產(chǎn)品編號(hào):AE437-25mg

產(chǎn)品規(guī)格:25mg

相關(guān)規(guī)格:
數(shù)量
價(jià)格 ¥150

Amphotericin B solubilized from Streptomyces sp

可溶性兩性霉素B

CAS Number: 1397-89-3  C47H73NO17  MW 924.08


產(chǎn)品編號(hào)

粉末含量

兩性霉素B有效含量

AE437-25mg

55mg

25mg

AE437-100mg

220mg

100mg


● Storage:2-8°C

● Specification:

Form…………………….fine, light yellow, lyophylized powder

Stock solution………… 2.5mg/ml(以有效含量計(jì)算) in H2O

● Product description:

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. After binding to sterols, it forms channels in the membranes, causing small molecules to leak out. Amphotericin B is effective against fungi and yeast. Normal usage for maintenance of cell cultures is 2.5 mg/l with penicillin and streptomycin in the medium. For cultures already contaminated with yeast and fungus, it is recommended to use this product at 2-4 times the normal level (5-10 mg/l), without penicillin and streptomycin for 2-3 subcultures. Once the infection is under control, normal maintenance levels of amphotericin B should be used. Amphotericin B is not soluble in H2O but in DMSO. Amphotericin B solubilized is a colloidal suspension of Amphotericin B, using sodium deoxycholate as the solubilizing agent. The product is approximately 45% amphotericin B, 35% sodium deoxycholate; the balance being sodium phosphate and sodium chloride.

● Preparation instructions:

Amphotericin B solubilized is reconstituted at the water in a 2.5 mg/ml stock solution. The solution must be filtered to sterilize by 0.22μm membrane.

● Storage/Stability:

The lyophylized powder amphotericin B solubilized can be stable for 5 years under -20℃ protected from air and light. Stock solutions at 2-8 °C are stable for up to 1 month.

● Reference:

1. Bolard, J., Biochim. Biophys. Acta, 864: 257-304 (1986).

2. Venegas, B., et al., Biophys. J., 85, 2323-32 (2003).

3. Vertut-Doi, A., et al., Biochem. Biophys. Res. Commun., 157, 692-97 (1988).

4. Sau K., et al., J. Biol. Chem., 278, 37561-68 (2003).

5. Perlman, D., Methods Enzymol., 58, 110-16 (1979).



 
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